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. 2012 Dec 14;7(12):e52293. doi: 10.1371/journal.pone.0052293

Figure 4. Sequence alignment of spacer regions.

Figure 4

The spacer sequences are aligned using ClustalW2 and amino acid residues are shown in different colors. X indicates missing amino acid residues and Gaps (–) have been inserted in order to align the amino acid residues. Positions with identical residues in a majority of the proteins are boxed. α-Helices and strands predicted by PSI-Pred v3.0 are shaded in red and yellow, respectively. (A) The sequences shown are the following: MiSp-Spacer (Ds), D. spinosa minor ampullate spidroin (GenBank accession no. ABD61589); MiSp-Spacer (Na), Nephila antipodiana minor ampullate fibroin 1 (GenBank accession no. ABC72645); MiSp-Spacer (Nc), N. clavipes minor ampullate silk protein MiSp1 (GenBank accession no. AAC14589); MiSp-Spacer (Ncr), Nephilengys cruentata minor ampullate spidroin-like protein (GenBank accession no. ABR37278); MiSp-Spacer (Aar), Argiope argentata minor ampullate spidroin (GenBank accession no. AFM29835); ADF1-Spacer (Ad), A. diadematus fibroin-1 (ADF-1) (GenBank accession no. AAC47008); MiSp (Av), A. ventricosus minor ampullate spidroin (GenBank accession no. JX513956). (B) The Nephila Flag-spacer sequences shown are the following: Flag-Spacer1–4 (Nc), N. clavipes flagelliform silk protein (Flag) (GenBank accession no. AAF36090); Flag-Spacer1–4 (Nc-U), N. clavipes flagelliform silk protein (Flag) (GenBank accession no. AAF36090); Flag-Spacer (Nc-D), N. clavipes flagelliform silk protein (Flag), downstream (GenBank accession no. AAF36089); FlagA-Spacer (Nc), N. clavipes flagelliform silk protein (Flag) (GenBank accession no. AAC38846); FlagB-Spacer1–2 (Nc), N. clavipes flagelliform silk protein (Flag) (GenBank accession no. AAC38847); Flag-Spacer1–4 (Nim), N. inaurata madagascariensis flagelliform silk protein (Flag) (GenBank accession no. AAF36091); Flag-Spacer1–4 (Nim-U), N. inaurata madagascariensis flagelliform silk protein (Flag) (GenBank accession no. AAF36091); Flag-Spacer 1–2(Nim-D), N. inaurata madagascariensis flagelliform silk protein (Flag), downstream (GenBank accession no. AAF36092); Flag-Spacer1–3 (Ncr), N. cruentata flagelliform spidroin-like protein (GenBank accession no. ABR37273). (C) The Argiope and Araneus Flag-spacer sequences shown are the following: FlagA-Spacer1–2 (At), A. trifasciata flagelliform silk protein (Flag) (GenBank accession no. AF350265_1); FlagB-Spacer1–3 (At), A. trifasciata flagelliform silk protein (Flag) (GenBank accession no. AF350264_1); Flag1-Spacer1–2 (Av), A. ventricosus flagelliform silk protein-1 (Ff1) (GenBank accession no. AAT36347); Flag-Spacer (Av), A.ventricosus flagelliform fibroin (GenBank accession no. ABK00016).