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. 2012 Dec 14;7(12):e52276. doi: 10.1371/journal.pone.0052276

Figure 3. B. pseudomallei are resistant to C3 deposition by the alternative pathway of complement.

Figure 3

B. pseudomallei and B. thailandensis were incubated with the indicated serum concentrations in the presence or absence of MgEGTA or EDTA, washed, and stained with an anti-human C3 FITC-conjugated antibody. Samples were analyzed by flow cytometry and the data reported as mean fluorescence intensity (MFI). The bars represent the mean±SEM of three separate experiments performed with duplicate samples. The single asterisk (*) indicates a statistically significant increase (P≤0.05) over unopsonized bacteria. The double asterisks (**) indicate a statistically significant difference (P≤0.05) between B. pseudomallei and B. thailandensis values.