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. 2012 Dec 14;7(12):e52276. doi: 10.1371/journal.pone.0052276

Figure 4. Levels of endogenous Burkholderia-reactive antibodies present in normal human serum.

Figure 4

B. pseudomallei, B. thailandensis, and E. coli were incubated with the indicated serum concentrations, washed, and stained with both an APC-labeled anti-human IgM antibody and R-PE-labeled anti-human IgG antibody. Samples were analyzed by flow cytometry and the data reported as mean fluorescence intensity (MFI). The bars represent the mean±SEM of two separate experiments performed with duplicate samples. The single asterisk (*) indicates a statistically significant increase (P≤0.05) over unopsonized bacteria. The double asterisks (**) indicate a statistically significant difference (P≤0.05) over 20% NHS-opsonized B. pseudomallei and B. thailandensis values.