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. 2012 Dec 14;7(12):e52276. doi: 10.1371/journal.pone.0052276

Figure 7. Serum opsonization enhances internalization and clearance of B. pseudomallei and B. thailandensis by neutrophils.

Figure 7

Isolated human neutrophils were treated with DPI or DMSO (vehicle control) for 20 min prior to bacterial infection. Neutrophils were incubated with B. pseudomallei and B. thailandensis for (A) 10 min to measure bacterial uptake or for (B) 2 h to measure bacterial survival. The bars represent the mean±SEM of three separate experiments using neutrophils from different donors and performed in duplicate. The single asterisk (*) indicates a significant difference (P≤0.05) compared to unopsonized bacteria. The double asterisks (**) indicate a significant difference (P≤0.05) between B. pseudomallei and B. thailandensis. The triple asterisks (***) indicate a significant difference (P≤0.05) between 20% NHS opsonized Burkholderia species and all other conditions.