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The open reading frames are colored according to presumed function: red, plasmid replication and partition; purple, conjugative transfer (tra); blue, mating pair formation (trb); green, transposon associated; gray, hypothetical proteins of unknown function. The origin of replication (oriV) and the origin of transfer (oriT) are indicated by black circles. An inner circle marks HindIII restriction sites (H) used in subcloning.
