Figure 9. Binding and the activation of the OsGS1;2, GAT1 and GAD3 promoters by OsMYB55 Electrophoretic Mobility Shift Assay (EMSA).

Binding of different concentrations of OsMYB55 protein to 200 ng of DNA containing one copy of the MB-box (A). Transcription activation was carried out by using GUS based transient assay and 4-methylumbelliferyl b-Dglucuronide (MUG) as the substrate (B). The 4-methyl umbelliferone (MU) was used to generate the standard curve. Bars represent mean ± SE. (n = 6).