Table 2. Non-biopsied tumors histology and map characteristics.

Patient #	delayed enhancement population	Histological description*	Tumor type
3	Overall enhancing lesion consists of71% blue population and22% red population	Cellular tumor with many mitoses and regions of “geographic necrosis”. In some regions of the tumor it is also possible to see proliterative blood vesselsSome regions surrounding the tumor (not in all slices and not all around the tumor) depict brain tissue infiltrated by a small number of tumor cells. The main findings in these regions around the tumor are abnormal proliferation of blood vessels and many histiocytes	GBM post chemoradiation
21 Metastasis #2	lesion consisting of blue (58%)regions and red (31%) regions	Metastatic carcinoma showing squamoid features with extensive areas of tumor necrosis	NSCLC cortical brain metastasis
22	lesion consisting of blue (56%)regions and red (35%) regions	Several samples showing regions of tumor and regions of radiation necrosis. Significant cauterize artifacts are noticed as well	Adenoid Cystic Carcinoma Cortical brain metastasis
23 Metastasis #1	Metastasis consisting of a centralred (42%) region surrounded by athick blue rim (47%)	Central slice shows a large necrotic region surrounded by significant regions of morphologically active tumor	NSCLC mediall brain metastasis Pathology report addressing metastases resected unblock
23 Metastasis #2	lesion consisting of blue (56%)regions and red (33%) regions	Morphologically active tumor is present in the histological samples	NSCLC cortical brain metastasis
24	lesion consisting of a large blue (56%)region surrounded by a red rim (34%)	Active tumor was found	Melanoma midline brain metastasis
25	lesion consisting of a blue central region(61%) surrounded by a red (33%) region	Active tumor was found	Breast cortical brain metastasis
26	lesion consisting of thin blue rim(40%) within a larger red (51%) mass	Large mass of radiation necrosis. Small fociof active tumor were found after ki67 staining	Breast peri-ventricular brain metastasis
28	lesion is mostly red (53%) with smallelongated blue regions (27%)	Mostly necrotic samples with small foci of active tumor	NSCLC medial brain metastasis

List of tumors with no stereotactic biopsies, delayed enhancement subtraction map characteristics and histological evaluation of 1 patient with primary brain tumor and 7 patients with brain metastases.

^*Pathology report is based on all samples obtain from the neurosurgeons unrelated to the pre-surgical maps.