Table 1. Primer information.
gene | Primer | sequence 5′ ⇒ 3′ | expected amplicon |
HPRT | qhHPRT.fwu1 | GACCAGTCAACAGGGGACAT | 95 bp |
qhHPRT.rvu1 | GTGTCAATTATATCTTCCACAATCAAG | ||
TATA-box | qTBP.fw1 | TGTGCACAGGAGCCAAGAGT | 51 bp |
binding protein | qTBP.rv1 | ATTTTCTTGCTGCCAGTCTGG | |
insulin | qhINS.fw1 | GCAGCCTTTGTGAACCAACA | 71 bp |
qhINS.rv1 | CGTTCCCCGCACACTAGGTA | ||
glucagon | qhGCG.fw1 | GCTGCCAAGGAATTCATTGC | 94 bp |
qhGCG.rv1 | GTCTGCGGCCAAGTTCTTCA | ||
somatostatin | qhSST.fw1 | CTGTGTCACCGGCGCTC | 123 bp |
qhSST.rv1 | TTGGGTTCAGACAGCAGCTCT | ||
Ucn 3 | qhUcn3.fw1 | GAGGGAAGTCCACTCTCGGG | 137 bp |
qhUcn3.rv1 | TGTTGAGGCAGCTGAAGATGG |