Figure 1. Apoptosis detection by propidium iodide (PI) staining of hypodiploid nuclei after IEC-6 incubation with NIV or DON (0.5–80 µM) for 24 h.

Both NIV and DON exibited a significant and concentration-related pro-apoptotic effect on IEC-6 at concentrations higher than 5 µM and 10 µM respectively (***P<0.001 vs control). NIV at the concentrations between 5 and 80 µM exibits a stronger pro-apoptotic effect compared to the same concentrations of DON (°°°P<0.001, °°P<0.01 vs DON). Data are expressed mean ± s.e.m. from at least three-independent experiments.