Figure 2. Apoptotic nuclear morphological changes highlighted by DAPI staining in cells treated with graded concentration of NIV and DON (0.5–20 µM) for 24 h.

Apoptotic cells showed pyknotic nuclei and apoptotic bodies were clearly identified after incubation with higher concentration of NIV while DON induces a less marked effect. Cultures were examined and photographed using a confocal microscope as described in method section.