Table 2. Lateral and axial resolutions measured with the 20× and 40× magnification objective lenses, respectively, on fluorescent microbeads or, for second harmonic generation, on collagen fibers within lymph nodes.

	20×, NA 0.95, WD = 2 mm
	imaging depth/µm	fluorescence			imaging depth/µm	SHG
		PMT	CCD	SI		PMT	CCD	SI
lateral	0	359	340	267	5	365	384	251
/nm		±19	±29	±8		±22	±31	±23
	100	1451	1475	1128	45	744	738	560
		±49	±56	±39		±34	±28	±26
axial	0	1369	1341	572	5	1558	1536	636
/nm		±50	±75	±32		±84	±58	±25
	100	4386	4453	1836	85	3215	3255	1056
		±200	±123	±121		±130	±130	±70