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. 2012 Dec 14;7(12):e51242. doi: 10.1371/journal.pone.0051242

Figure 4. Phospho-GFP-SET-S171A levels in Jurkat were unchanged by PHA stimulation with or without PKD2 inhibitor.

Figure 4

The Jurkat cells expressing GFP-SET-S171A were stimulated and phosphorylation status of GFP-SET-S171A was investigated by the same method as described in Fig. 2. The results were indicated as mean +SD for three independent experiments. There was no significant up- or down-regulation of recovered phospho-GFP-SET-S171A by PHA stimulation with or without PKD2 inhibitor, suggesting that Ser171 of SET is the major target site of TCR-activated PKD2.