Figure 7. Overexpression of GFP-SET-S171A reduced the ERK activation after stimulation.

Normal and GFP-SET-S171A-overexpressing Jurkat cells were stimulated by anti-CD3e antibody and anti-mouse IgG antibody for 7 min were lyzed and subjected to SDS-PAGE followed by the western blot analyses. The phospho-ERK (p-ERK) was first detected and quantified and after stripping off the antibodies, ERK1 was detected and quantified to monitor p-ERK/ERK1 ratio. The p-ERK/ERK1 ratio of Jurkat cell was assigned to be 1. The results were indicated as mean +SD for three independent experiments. *, p<0.01, as determined by Student's t test.