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. 2012 Dec 14;7(12):e51399. doi: 10.1371/journal.pone.0051399

Figure 2. Northern hybridizations detecting P. infestans antisense sRNAs derived from transposons, RxLR and CRN effector genes in R0 and 3928A isolates.

Figure 2

A. sRNAs hybridizing to Copia3-LTR, PITG_22969 (CRN), Crypton6, PiAvrblb1 (RxLR) were detected. Loading controls (U4 spliceosomal RNA) are shown below each autoradiograph. B. Determination of 5′ terminal modifications to sRNAs. From left, terminator exonuclease (TE) and tobacco acid pyrophosphatase (TAP) treatment of 21 and 32 nt sRNAs from Copia 3-LTR and Crypton6, respectively in isolate R0. C. ß-elimination assay for 3′ modifications to sRNAs, determined for 21 nt sRNAs for Copia3-LTR in isolate R0. Lane +β is after treatment with periodate, showing a 1 nt downward shift in size compared to the untreated sample (−β).