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. 2012 Dec 14;7(12):e51773. doi: 10.1371/journal.pone.0051773

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© 2012 Coppens et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) IFA of Toxoplasma coexpressing CAV1-YFP cavin-1-HA immunolabeled with antibodies against HA (cavin-1-HA) and SAG1 (plasma membrane, blue) showing no overlap with the plasma membrane. (B) Assessment of Alexa Fluor 594- CT-B distribution after 30 min-pulse by fluorescence microscopy in cavin-1-HA-expressing parasites (panel a) and CAV1-YFP- and cavin-1-HA-expressing Toxoplasma (panels b and c), showing no internalization of the CT-B regardless of the proximity of the structures containing CAV1 or cavin-1.