Figure 1. Infection of susceptible pepper leaves with Xcv wild type or with the T3SS-deficient Xcv ΔhrpB1 strain.

Impact on symptom development and transcript accumulation of cw-Inv, PRQ and RbcS. Fully mature leaves of young pepper plants were infected with the Xcv wild type (wt), the Xcv ΔhrpB1 using a concentration of 5×10⁸ cfu ml⁻¹, and as control with 10 mM MgCl₂. A.) Formation of disease symptoms after Xcv wild type inoculation of susceptible pepper leaves. Only the lower halves of leaves were infiltrated. Pictures were taken 3 days post infection. B.) For Northern Blot analysis total RNA was isolated from leaf material taken before (0 h) and 3, 6, 12, 18, 24 and 48 hours post infection (hpi). Thirty micrograms of total RNA were loaded per each lane. The Northern blot was probed with [³²]P-labeled cDNA fragments of cw-Inv, PRQ, RbcS and cytosolic GAPDH as control. Results of a representative experiment are shown which have been repeated three times.