Figure 5. Cw-Inv activity of susceptible pepper leaves infected with heat-inactivated and protease-treated preparations of Xcv cells.

Xcv wild type (wt) and Xcv ΔhrpB1 were grown over night in NYG medium and prepared as described in material and methods. Subsequently, cells were heat-inactivated at 95°C for 20 min. An aliquot was of each cell culture was digested with proteases (proteinase K and trypsin) for 2 h at 60°C. After heat-inactivation for 10 min, cells were pelleted and re-suspended in 10 mM sterile MgCl₂ and adjusted to OD₆₀₀ = 1. Heat-inactivated Xcv cells with or without protease treatment were used for inoculation of pepper leaves. Samples were taken before (0 d), 1, 2 and 3 days post infections (dpi) and cw-Inv activity was measured. Values represent the mean of four independent samples ± SD. Similar results were obtained in an independent experiment.