Figure 9. Expression of XopB in Xcv ΔxopB complements the effect on cw-Inv activity.

Susceptible pepper leaves were inoculated with Xcv wild type (wt), Xcv ΔxopB containing both the pBBR1MCS5 vector (EV), or Xcv ΔxopB derivatives in which a genomic fragment was introduced containing the putative xopB promoter and open reading frame in sense (+) or antisense (−) orientation relative to the lac promoter. Samples were taken before (0), 1, 2 and 3 days post infection (dpi). A.) Cw-Inv activity was measured from the infected leaf tissue. Values represent the mean of four independent samples ± SD. B.) Expression of XopB was verified by Western blotting by probing with the anti-XopB antibody. XopB migrates at ∼70 kDa.