Figure 11. Inducible xopB expression in transgenic tobacco leaves suppresses cw-Inv activity during Xcv infection.

Control plants and two selected transgenic tobacco lines with inducible xopB expression (#22, #71) were watered with 1% ethanol. After 24 h, plantlets were inoculated with a 10⁹ cfu ml⁻¹ suspension of Xcv wild type. Samples were taken directly before inoculation and 1 and 2 days post inoculation (1dpi +EtOH; 2dpi Xcv+ EtOH). Non-ethanol watered plants were also inoculated with Xcv and samples were taken accordingly (1dpi -EtOH; 2dpi -EtOH). For control purposes ethanol-treated and non-treated plants were inoculated with 10 mM MgCl₂. Cw-Inv activity was determined from four independent samples and fold changes ± SD were calculated for each sample relative to values obtained before Xcv inoculation. The experiment was repeated with similar results.