Figure 10. Mtb Δfcr mutants show increased metabolic activity and induction of genes involved in energy generation and transcription under dormancy-inducing conditions.

A, Mtb WT and fcr deficient cells subjected to combined MS condition for 0, 9 and 18 days were incubated with Alamar Blue dye for indicated periods of time and fluorescence was measured using a plate reader. B, Relative gene expression values (fold induction of day 0) of each gene for WT and Δfcr mutants at 9 and 18 days under MS. Real-time Taqman RT-PCR measurement was performed to measure relative abundance of transcripts. Relative quantitation method (ddCt) was used with the 7900 HT real-time system and analysis was done using SDS v2.3 software of Applied Biosystems Inc. Samples of starter culture (day 0) were used as calibrator and sigA was used as the endogenous control to normalize expression values.