Figure 4. Being reared in a CN, independent of birth order, increased adult affiliative behavior and OTR binding, compared to the SN condition.
(A) Affiliative behavior measured on the fifth agonistic encounter when the social hierarchy is established. Old, Middle (Mid) and Young (Yng) groups (respectively, n =20, 19 and 20) showed a higher propensity to interact with a conspecific compared to standard nesting (SN) mice (n =18). (B) OTR binding. Old, Middle and Young mice (respectively, n =10, 9 and 10) showed higher levels of oxytocin receptor binding in the ACo and the CeA, compared to SN mice (n= 9). ACo, anterior cortical nucleus of the amygdala; CeA, central amygdala; LA, lateral amygdala; BLA, basolateral amygdala. The cage was considered as the statistical unit.* and ** = respectively p < 0.05 and < 0.01 vs. SN mice. Data shown are means ± SEMs.