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. Author manuscript; available in PMC: 2014 Feb 22.
Published in final edited form as: Oncogene. 2012 Sep 10;32(34):4043–4051. doi: 10.1038/onc.2012.400

Figure 2. GPX1 Expression and TFAP2C regulation.

Figure 2

A. GPX1 expression by RT-PCR (top panel) and Western blot (bottom panels) in four breast cancer cell lines. B. GPX1 expression in BT-474 and MDA-MB-453 after transfection with siRNAs: Mock (mock transfected), non-targeting (NT), TFAP2A (A) or TFAP2C (C) or both siRNAs (A+C). C. The 60 bp region of the GPX1 gene promoter with consensus sequences of potential AP-2 binding sites (underlined) and relative location of additional oligonucleotide competitors used in gel-shift assay. D. Gel shift with radiolabeled probe shown in C with oligo competitors. The locations of free probe, TFAP2C complex and complex supershift are noted.

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