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. Author manuscript; available in PMC: 2014 Feb 22.
Published in final edited form as: Oncogene. 2012 Sep 10;32(34):4043–4051. doi: 10.1038/onc.2012.400

Figure 3. GPX Activity and Sensitivity to tert-butyl hydroperoxide.

Figure 3

A. Selenium-dependent GPX activity in four breast cancer cell lines demonstrated significantly greater activity in BT-474 and MDA-MB-453 compared to MCF-7 and SKBR-3. B. Sensitivity to tert-butyl hydroperoxide was measured at various concentrations comparing BT-474 transfected with siRNA to TFAP2C (siTFAP2C) or a non-targeting siRNA (siNT) and demonstrates increased drug sensitivity with knock down of TFAP2C over a concentration range of 20 to 60 μM. C. Selenium-dependent GPX activity was induced in MCF-7 cells by overexpression of GPX1 by infection with Ad-GPX1 (G) compared to Ad-empty (E) or Ad-TFAP2C (C), p<0.05. Western blots below graph confirm expression of proteins after viral infection. D. Sensitivity to tert-butyl hydroperoxide was measured at various concentrations comparing MCF-7 cells infected with adenoviruses indicated demonstrating that overexpression of GPX1 significantly increased resistance to tert-butyl hydroperoxide at concentrations of 40 μM and above.

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