Figure 5. Methylation of GPX1 gene and expression of GPX1, TFAP2A and TFAP2C in primary breast cancer samples.

A. Examples of results from bisulfite sequencing demonstrating the methylation patterns among primary breast cancer samples showing high degree of methylated (1–4) and unmethylated (5–8) chromatin. B/C. Semi-quantitative RT-PCR used to determine a correlation between GPX1 expression and expression of TFAP2A (B) and TFAP2C (C) in panel of primary breast cancer samples (n=20). Results demonstrated strong association between expression of TFAP2C and GPX1 in unmethylated samples with R²= 0.908, p<0.0001. No significant correlation was found comparing GPX1 expression with TFAP2A expression with R²=0.022, p=0.58.