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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Hepatology. 2012 Dec 6;57(2):786–796. doi: 10.1002/hep.26056

Figure 4. KLF6WT transcriptionally inhibits Colα1(I),Colα2(I), and β-Pdgfr; both KLF6 isoforms lead to increased apoptosis of stellate cells in vitro.

Figure 4

A. Mouse stellate cells were isolated from Colα2(I)-KLF6WT, Colα2(I)-KLF6SV1, and wild type mice (5 mice each) following 3 doses of CCl4. A decrease in fibrogenic gene expression in Colα2(I)-KLF6WT, but not Colα2(I)-KLF6SV1 transgenic stellate cells is evident in fresh HSC isolates; B. Increased apoptosis in human stellate cell lines (LX2) following stable transfection of pBabe-KLF6WT, or pBabe-KLF6SV1, based on the presence of cleaved form of PARP; C. Immortalized mouse stellate cells (JS1) transfected with Colα2(I)-KLF6WT , or Colα2(I)-KLF6SV1 constructs, and samples were prepared for chromatin IP 48h after transfection. Primer sets specific for GC boxes in promoter regions (2kb region upstream of start site) of Colα1(I), Colα2(I), and β-Pdgfr demonstrate binding of KLF6WT, but not KLF6SV1 to all three promoters; D. mouse stellate cells (JS1) over-expressing pBabe-KLF6WT show decreased transcript levels of Colα1(I), Colα2(I), and β-Pdgfr (p-value<0.05).