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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: J Am Coll Cardiol. 2012 Nov 14;60(24):2527–2534. doi: 10.1016/j.jacc.2012.09.023

Figure 4. Ex vivo validation.

Figure 4

From TTC-stained macroscopic short axis slices (A.), samples for infarct (INF), remote left ventricle (RMT) and right ventricle (RV) were taken. (B.) Real-time PCR shows increased expression of angiotensin II type 1 receptor (AT1R), relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping control (*, p<0.05 vs control; ¶, p<0.001 vs others). (C.) Histology shows tissue damage and presence of inflammatory cells in infarct compared to remote at HE staining (left), and presence of fibrosis in the infarct region at Masson-trichrome staining (middle). Immunostaining for AT1R (right) shows a positive, brown-colored signal from spindle-type cells in the infarct (white arrows), while myocytes in the remote region also show positive signal, indicated by diffuse, brown-colored staining.