Table 1.

Melting Temperatures and van’1t Hoff Thermodynamic Parameters for RNA and DNA G-Quadruplex-Forming Libraries^a and Naturally Occurring RNA G-Quadruplex-Forming Sequences^b

	Tm(VH)/Tmc (°C)	ΔHvh (kJ mol−1)	ΔSvh (kJ mol−1 K−1)	ΔGvh(37 °C) (kJ mol−1)
L111	86/86	−249 ± 16	−0.692 ± 0.045	−33.8 ± 1.8
L112	78/78	−237 ± 10	−0.674 ± 0.030	−27.6 ± 1.2
L121	76/76	−239 ± 15	−0.685 ± 0.042	−26.7 ± 1.4
L211	77/77	−247 ± 4	−0.703 ± 0.013	−28.3 ± 0.1
L113	74/74	−241 ± 17	−0.701 ± 0.067	−25.7 ± 1.4
L131	73/73	−279 ± 16	−0.798 ± 0.064	−25.7 ± 1.6
L311	75/75	−238 ± 2	−0.684 ± 0.007	−26.0 ± 0.1
L122	72/72	−266 ± 17	−0.768 ± 0.075	−26.5 ± 1.0
L212	72/72	−245 ± 1	−0.711 ± 0.002	−24.6 ± 0.8
L221	70/71	−251 ± 1	−0.730 ± 0.006	−24.4 ± 0.3
L222	67/67	−236 ± 19	−0.693 ± 0.056	−20.8 ± 1.6
L133	63/63	−242 ± 5	−0.721 ± 0.014	−18.4 ± 0.2
L313	65/65	−233 ± 4	−0.687 ± 0.012	−19.2 ± 0.6
L331	63/64	−243 ± 0	−0.722 ± 0.000	−19.1 ± 0.1
L223	63/63	−241 ± 5	−0.717 ± 0.017	−18.6 ± 0.4
L232	61/62	−238 ± 14	−0.712 ± 0.041	−17.4 ± 1.2
L322	59/59	−224 ± 4	−0.673 ± 0.012	−14.9 ± 0.2
L233	59/59	−231 ± 3	−0.693 ± 0.008	−15.4 ± 0.5
L323	59/59	−218 ± 9	−0.657 ± 0.027	−14.2 ± 0.3
L332	57/58	−220 ± 6	−0.665 ± 0.016	−13.5 ± 0.6
L333	55/55	−232 ± 7	−0.707 ± 0.021	−12.9 ± 0.6
L444	49/50	−159 ± 0	−0.493 ± 0.001	−6.1 ± 0.1
L555	46/47	−165 ± 4	−0.518 ± 0.013	−4.6 ± 0.1
dL111	77/78	−190 ± 9	−0.543 ± 0.027	−21.9 ± 0.6
dL222	41/41	−116 ± 3	−0.368 ± 0.008	−1.4 ± 0.1
dL333	38/38	−132 ± 1	−0.425 ± 0.004	−0.3 ± 0.0
TRF2	77/78	−255 ± 6	−0.728 ± 0.016	−29.4 ± 0.9
MT3	71/71	−247 ± 6	−0.719 ± 0.016	−24.1 ± 1.0
NRAS	69/68	−244 ± 4	−0.713 ± 0.012	−22.7 ± 0.5
TERRA	57/58	−265 ± 5	−0.799 ± 0.016	−16.3 ± 0.3

^aFor the libraries, these measurements represent apparent values derived from melting curves of the mixtures.

^bAll errors are given as the standard deviation. T_m values are accurate to ±1 °C.

^cT_m(VH) values are melting temperatures determined from van’t Hoff analysis, whereas T_m values are determined as the point at which the folded fraction equals one-half.