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. 2012 Sep 28;1(12):1204–1214. doi: 10.1242/bio.20122659

Fig. 7. Sequential phosphorylation and dephosphorylation of GRASP65 orchestrate mitotic Golgi disassembly and post-mitotic reassembly.

Fig. 7.

NRK cells of indicated cell cycle phases were triple labeled by (A) LX108 (T220/T224), R3G3 (S277) and Mary (total GRASP65 regardless of the phosphorylation state) and (B) LX108, RB7 (S376) and Mary. Cell cycle stage was determined by the morphology of the DNA. Note that the intensity of LX108 signal correlates with the progress of Golgi disassembly. Scale bar in all panels: 10 µm.