Figure 6.

Aaop1 is expressed in most R8 photoreceptor cells. A, A retina at ZT14 is imaged for Aaop1 (green) and actin (red). The white line identifies the border between the dorsal region in which the R8 cells lack Aaop1 expression and the rest of the retina in which R8 cells express Aaop1. B, The boxed region in A is enlarged to highlight ommatidia in the border region to distinguish the two classes of ommatidia. The top three rows of ommatidia lack Aaop1 expression in the central R8 rhabdomere, whereas the lower two rows show Aaop1 expression in the R8 rhabdomere. C, D, A z-plane section through the ommatidia identified by the line in B. Arrows point to the area occupied by the R8 rhabdomere. The R8 rhabdomere is Aaop1-positive in the ommatidia from the section shown in D but not in the ommatidia from the section shown in C. The asterisk in D identifies the area of the rhabdom occupied by the R7 rhabdomere. E, A z-plane section of ommatidia from a retina at ZT13, immediately after the dusk dimming period. At this time point, the R8 cell body located centrally below the rhabdom contains many Aaop1-labeled cytoplasmic structures (arrow).