Figure 3.

Cell migration and wound healing assays. (a) The effect of M/D-CTX-Fcs on the migration of A172 cells was assessed using a PET track-etched membrane culture insert (pore size, 8.0 μm). The cells were incubated with M/D-CTX-Fcs in the range of 0–300 nM. Translocated cell numbers were normalized against those in the absence of CTX. The results are shown as means ± SD. (b) The inhibition of cell migration by M/D-CTX-Fcs was assayed by wound healing. The effect on migration was evaluated based on the change in the distance. The data (mean ± S.D) presented are from three independent experiments. (*P < 0.1, **P < 0.05, ***P < 0.01).