Expression of p53 isoforms in tumour tissue (T), clinically normal tumour adjacent tissue (N) in patients with squamous cell carcinoma of the head and neck, and healthy non-smoking controls (NS) detected by nested RT-PCR. (a) Detection of p53, size of the amplified fragment was - 1250 bp. (b) Detection of p53β, size of the fragment was - 1050 bp. (c) Actin is shown as control. X indicates samples showing variable results in duplicate analysis that could not be classified as either positive or negative. M indicates molecular weight marker. p53, p53β and actin are amplified from the same cDNA samples, T5 has lower actin in this run but in the duplicate all samples have the same actin level.