Figure 4.

Utilization of carbon sources by Y. pseudotuberculosis wildtype and the crp mutant. The 96-well BIOLOG plates PM1 (A) and PM2 (B) contain a minimal medium with trace elements except that the C-source is omitted. The various wells in these panels provide 190 different carbon sources. The assays are initiated with the addition of bacterial culture. After incubation, respiration of the bacteria leads to the reduction of a tetrazolium dye, indicating that the bacteria are metabolically active. Information about the carbon sources supplied in the wells is given on the manufacturer’s home page (http://www.biolog.com/pdf/pm_lit/PM1-PM10.pdf). Utilization of the metabolites by the wildtype strain YPIII is given by the green areas in the growth curves and use of the carbon sources by the crp mutant YP89 is indicated by the red areas of the growth curves. The data represent one experiment of three independent analyses.