Figure 6.

Global influence of Crp on gene expression and metabolism of Y. pseudotuberculosis. (A) Differentially regulated genes between the wildtype and the crp mutant. Sixteen independent cultures of strains YPIII and YP89 were grown in LB medium at 25°C to early stationary phase, total RNA was isolated and four samples were pooled for RNA-labeling with Cy5 (wildtype) and Cy3 (Δcrp). The mixed RNA probes were hybridized to an Agilent microarray carrying 4172 chromosomal and 92 virulence plasmid encoded genes of Y. pseudotuberculosis. After normalization, changes in gene expression are given as median values for at least three probes (Table S1 in Supplementary Material). Genes showing overall fold-changes ≥1.8 are included in the displayed diagram of gene classes. (B) Central carbon metabolism in Y. pseudotuberculosis. Significant alterations in the metabolic and transcriptomic pattern between the wildtype and the crp mutant are indicated. An increase of a metabolite in the crp mutant is indicated by showing the metabolite in red (Crp-repressed), whereas a decreased metabolite is displayed in blue (Crp-activated). Accordingly, upregulated genes in the crp mutant are indicated in red (Crp-repressed), downregulated genes are given in blue (Crp-activated).