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. Author manuscript; available in PMC: 2013 Dec 11.
Published in final edited form as: Dev Cell. 2012 Dec 11;23(6):1103–1110. doi: 10.1016/j.devcel.2012.10.003

Figure 3.

Figure 3

Noise in STORM/PALM. (A) Zoomed-in view of a boxed region in Figure 2B, left panel, showing false localization points (arrows). Particularly, misidentification of two nearby fluorophores activated at the same time creates the false localization points in the region surrounded by three microtubules. (B) Inset of Figure 1A before correction for crosstalk. The crosstalk can be seen as mixed green and red localization points. Arrows point to localization points either from nonspecific antibody binding or from false identification of background noise.