(A) Movie montage shows EB1 comets grow into branches that extend and remain stable in a wild type neuron (arrow indicates point of branch extension within 30 min) (See also Figure S5). (B) Quantification of the correlation between EB1 comet growth within a branch and branch dynamics; n = 86 branches. (C) Representative images of dendrite branch dynamics in wild type or γ-tubulin23C RNAi/γ-tubulin23C(A15-2) neurons within 30 min. Arrows, arrowheads, and stars indicate points of branch extension, retraction, and stability after 30 min for each genotype. (D) Quantification of total branch dynamics in wild type, γ-tubulin23C RNAi/γ-tubulin23C(A15-2) and CP309(5)/CP309(5) neurons. N = 96, 101, and 120 branches for wild type, γ-tubulin mutant, and CP309 mutant, respectively.