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. Author manuscript; available in PMC: 2013 Dec 5.
Published in final edited form as: Cell Metab. 2012 Dec 5;16(6):801–813. doi: 10.1016/j.cmet.2012.10.018

Figure 6. Mss51 mutants bypass the requirement of heme for efficient COX1 mRNA translation.

Figure 6

(A–E) In vivo mitochondrial protein synthesis in the indicated strains treated or not with DHA in the presence of TEM or in (D) the Δhem1, mss51T167R strain incubated in the presence of either ALA or TEM. In (E), following a 10 minute pulse, the labeled proteins were chased for 30 and 60 min. The signals were quantified as in Fig. 1B and used to plot the graphs in the lower or side panels.

(F) Sucrose gradient sedimentation analyses of GST-tagged Mss51F199I in mitochondrial extracts prepared from the indicated strains as in 2I.