Figure 2. Time- and dose-dependent effects of ethanol on negative geotaxis in control flies.
Negative geotaxis measured in eRING assays was significantly affected by ethanol concentration and time of exposure in female (A) and male (B) Canton-S (CS) control flies (two- way ANOVA, p<0.0001). (C) T50 values derived from data in panels A and B decreased as the ethanol concentration was increased (two-way ANOVA, p<0.0001), but there was no effect of sex (two-way ANOVA, ns). Data (mean ± S.E.M.) are compiled for n=15 vials (25 flies/vial) from three independent experiments.