Female flies were assessed for ethanol sensitivity (A), rapid ethanol tolerance (B), and internal ethanol concentrations (C). (A) T50 values were lower in flies heterozygous for mysts2 or scbvol2 (one-way ANOVA, p<0.001, *Bonferroni’s multiple comparison test, p< 0.05) and further reduced in mysts2;scbvol2 double heterozygous flies compared to control (**p<0.05). (B) The development of rapid ethanol tolerance was enhanced relative to w[CS] flies in mysts2 and scbvol2 heterozygotes and in mysts2/+; scbvol2/+ double heterozygotes (non-Gaussian data, Kruskal-Wallis one-way ANOVA, p<0.0001, *Dunn’s multiple comparison test, p<0.05). Data (mean ± SEM) were compiled for n=10 vials (25 flies/vial) from three independent experiments. All data are mean ± S.E.M.