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. Author manuscript; available in PMC: 2012 Dec 17.
Published in final edited form as: Oral Microbiol Immunol. 2005 Jun;20(3):131–140. doi: 10.1111/j.1399-302X.2004.00190.x

Fig. 4.

Fig. 4

Genetic analysis of the srpA ORF. A) Northern blot showing the srpA transcript from S. cristatus CC5A. Total RNA from wild-type CC5A was hybridized with a DNA sequence amplified by PCR from the 5′-end of the srpA gene. Molecular size standards are shown in kb. The arrow marks the position of a 11 kb hybridization positive band. B) Southern blot of the distribution of srpA DNA in various Streptococcus strains. Total genomic DNA was obtained from S. cristatus strains CC5A, CR3, Psha and Pshb; S. oralis CN3410; S. parasanguis FW213; and F. nucleatum ATCC 10953. The DNA samples were digested to completion with HindIII and the fragments hybridized with a fragment of DNA amplified from the 5′-end of the CC5A srpA ORF. The positions of molecular size markers are shown in bp.