Table 1.
Bacterial strains and plasmids used in this study
| Strain or Plasmid | Description | Source/Reference |
|---|---|---|
| Strains | ||
| S. cristatus CR311 | wild-type strain; type strain; fibrillar tufts | Laboratory collection |
| S. cristatus CC5A | wild-type strain; fibrillar tufts | Laboratory collection |
| S. cristatus Psha | wild-type strain; fibrillar tufts | Laboratory collection |
| S. cristatus Pshb | wild-type strain; fibrillar tufts | Laboratory collection |
| S. cristatus CR3 | wild-type strain; fibrillar tufts | Laboratory collection |
| S. cristatus CR311var1 | hydrophobic subpopulation of CR311 | This study |
| S. cristatus CR311var3 | hydrophilic subpopulation of CR311 | This study |
| S. oralis CN3410 | wild-type strain; fibrillar tufts | Obtained from H. Jenkinson (13) |
| S. parasanguis FW213 | fimbriate strain; expresses fap1 | Obtained from P. Fives-Taylor (7) |
| C. matruchotii NCTC 14266 | corncob partner | Laboratory collection |
| F. nucleatum ATCC 10953 | corncob partner | Laboratory collection |
| Escherichia coli JM109 | host strain for all plasmids | Promega |
| Plasmids | ||
| pB15-23 | pCR-Script SK+ contains 21 kb SalI fragment from S. cristatus B15 locus (Ampr) | (5) |
| pB15-18 | subclone of pB15-23 contains 10.9 kp EcoRI—Sau3A fragment (Ampr) | This study |
| pB15-2.2 | subclone of pB15-18 contains 2249 bp EcoRI – HindIII fragment cloned into pGem-T (Ampr) | (5) |
| pB15-1 | subclone of pB15-18 contains 1017 bp HindIII fragment (Ampr) | This study |
| pB15-8 | subclone of pB15-18 contains 7622 bp HindIII—Sau3A fragment (Ampr) | This study |
| Zap Express™ | lambda cloning vector | Stratagene |
| pBK-CMV | phagemid vector derived from ZAP Express™ (Kanr) | Stratagene |
| pY-1 | contains a fragment, with an additional 3895 bp adjacent to the Sau3A end of pB15-23; cloned into pBK-CMV (Kanr) | This study |
| pW-2 | contains a fragment with an additional 550 bp adjacent to pY-1 cloned into pBK-CMV (Kanr) | This study |