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. Author manuscript; available in PMC: 2012 Dec 17.
Published in final edited form as: Biotechniques. 2012 Jul;53(1):57–60. doi: 10.2144/0000113854

Figure 3. Flow cytometric characterization of mouse mammary epithelial cells stained by the modified method.

Figure 3

(A) Gating for live cells based on DNA content, separation of single cells by FSC width and SSC width, and gating for CD45-CD31- cells. (B) Pseudo color plot of CD24/CD49f expression, dot plot of CD24/CD29 expression, histogram of CD61 expression in luminal cells, pseudo color plot of CD24/CD44 expression, and histogram of DNA content for cell cycle analysis in CD45-CD31- mouse mammary epithelial cells. (C) Overlaid dot plots and histograms showing CD24/CD49f or CD24/ CD29, CD61, CD44, and CD49f expression in total CD45-CD31- cells (red) compared with actively dividing cells containing >2n DNA (black). Data shown are from one of six mice and three such independent experiments were carried out.