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. Author manuscript; available in PMC: 2012 Dec 17.
Published in final edited form as: Free Radic Biol Med. 2012 Jan 8;52(6):1075–1085. doi: 10.1016/j.freeradbiomed.2011.12.024

Fig. 8.

Fig. 8

Fragmentation ion spectra of [M + 2H]2+, [M + 138 + 2H]2+ (Cys-149 and Cys-153), [M + 32 + 2H]2+ (Cys-149), and [M + 48 + 2H]2+ (Cys-149) peptides from tryptic digests of HOSCN-treated GAPDH. (a) MS/MS spectrum of the doubly charged species m/z 912.0 (carboxymethyl peptide), with the fragment ions specific for the Cys-149 + 58 and Cys-153 + 58 product. Fragment ions are singly charged. Equivalent data are shown in (b), (c), (d), and (e) via monitoring of the doubly charged species m/z 952.0, 899.0, and 907.0, respectively. Data are representative of at least five independent experiments.