Table 2. Catalytic Efficiencies for dCTP Insertion with Pol β Utilizing Minor Groove Adducted BPDE-dG Adducted Templates.
| discrimination |
||||
|---|---|---|---|---|
| template (dG) | DNA gap | kcat/Km (M–1s–1) | fDNAa | fadductb |
| unadducted | – | 7.2 × 106 | 1.0 | 1.0 |
| C10S BPDE | – | 4.8 × 102 | 1.0 | 1.5 × 104 |
| C10R BPDE | – | 1.0 × 102 | 1.0 | 7.2 × 104 |
| unadducted | + | 8.3 × 107 | 11.5 | 1.0 |
| C10S BPDE | + | 8.2 × 102 | 1.7 | 1.0 × 105 |
| C10R BPDE | + | 1.2 × 102 | 1.2 | 6.9 × 104 |
a
fDNA = (kcat/Km)gapped/(kcat/Km)nongapped.
b
fadduct = (kcat/Km)unadducted/(kcat/Km)adducted.