Figure 5.

Sox2 and Cdx2 are co-expressed. IHC on sequential sections of E18.5 duodenum showed Sox2 expression only in double transgenic intestines (A), and Cdx2 expression throughout the epithelium in both control and double transgenic embryos (B). Confocal microscopy (C) of control and double transgenic animals showed that Cdx2 and Sox2 are co-expressed in the same intestinal epithelial cells in Sox2 expression embryos. Individual images of DAPI (blue), Cdx2 (green), and Sox2 (red) stainings are shown as inserts. Scale bar, 50 μm.