Figure 5.

Adoptive transfer of iTreg to lupus mice suppresses the expansion of DCs and decreases their B7 expression. 12 × 10⁶ fresh D2 CD4⁺ cells alone or together with 5 × 10⁶ CD4_con cells or iTreg were transferred into D2B6F1 mice. (A and B) CD80 and CD86 expression in each group of mice was examined 3 weeks later. ***P <0.001, iTreg vs. CD4_con cells. (C) Total splenic CD11c⁺ cells were counted. Data are representative or values are mean ± SEM of five mice in each experiment and combined from two independent experiments. (D) CD11c⁺ cells were sorted from each group of D2B6F1 mice as above and added to cultures containing CD25⁺-depleted T cells isolated from BALB/c mice for 3 days. T cell proliferation was determined as in above. Values are mean ± SEM of three separate experiments. ***P <0.001, DCs primed with iTreg vs. DC primed with CD4_con.