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Real-time PCR of p57 internal standard. Copy number profile of the p57 plasmid containing the 450-bp C. cladosporoides (Cc) insert was determined by qPCR. The first curve set is 106, tenfold serially diluted to 100. The copy number was determined by mass, and correlation coefficient of the curve is 0.996 (experiment was run in triplicate).
