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. 2013 Apr;24(1):32–38. doi: 10.7171/jbt.13-2401-004

TABLE 1.

Real-Time PCR Primers Used in This Study

Organism Primer Sequence (5′→3′) Tm (°C) Amplicon (bp) Set point (°C)
PCR insert
    C. cladosporoides Sense ATC ATT ACA AGT GAC CCC GGC 60 450 60
Antisense TTT ACG GCG TAG CCT CCC 58
rRNA
    A. alternata Sense CGA ATC TTT GAA CGC ACA TTG 57 163 57
Antisense CGC TCC GAA ACC AG TAG G 58
    A. fumigatus Sense CTG TCC GAG CGT CAT TGC 58 140 57
Antisense TAC AGA GCA GGT GAC AAA GC 58
    A. versicolor Sense GCG AAC TGC GAT AAG TAA TGT 57 111 57
Antisense GCA ATG ACG CTC GGA CAG 58
    C. cladosporoides Sense GCG TCA TTT CAC CAC TCA AG 58 128 57
Antisense CTC CCG AAC ACC CTT TAG 56
    Penicillium chrysogenum Sense CTG TCC GAG CGT CAT TGC 58 137 57
Antisense CAG AGC GGG TGA CAA AGC 58
    S. chartarum Sense GAG TAG TTT GGG AAT GCT GC 58 174 57
Antisense GCC CAA GTC TGG TCA TAA AC 58
β-Tubulin
    A. alternata Sense GCG AAC AAC AGA GAA CAA CAG 54 143 55
Antisense AAA GGC AGC ACC GAT TTG G 56
    A. fumigatus Sense GCT GGA GCG TAT GAA CGT C 56 165 55
Antisense GAC AGC GTC CAT GGT ACC 55
    A. versicolor Sense GCG AAC TGC GAT AAG TAA TGT 55 108 55
Antisense GCA ATG ACG CTC GGA CAG 56
    C. cladosporoides Sense GCA TAC ACC GAT TGA CAA C GCC 55 121 55
Antisense TTG GGA GTT CAT ACC 56
    P. chrysogenum Sense CGA TGG CGA TGG ACA GTA AG 55 131 55
Antisense ACT CAC ATG GTT GAA GTA GAC G 55
    S. chartarum Sense ACC TCT CGG CTC ACA ATT TC 55 165 55
Antisense TTG TTG CCA GCA CCA GAC 56

Tm, Temperature.