TABLE 1.
Organism | Primer | Sequence (5′→3′) | Tm (°C) | Amplicon (bp) | Set point (°C) |
---|---|---|---|---|---|
PCR insert | |||||
C. cladosporoides | Sense | ATC ATT ACA AGT GAC CCC GGC | 60 | 450 | 60 |
Antisense | TTT ACG GCG TAG CCT CCC | 58 | |||
rRNA | |||||
A. alternata | Sense | CGA ATC TTT GAA CGC ACA TTG | 57 | 163 | 57 |
Antisense | CGC TCC GAA ACC AG TAG G | 58 | |||
A. fumigatus | Sense | CTG TCC GAG CGT CAT TGC | 58 | 140 | 57 |
Antisense | TAC AGA GCA GGT GAC AAA GC | 58 | |||
A. versicolor | Sense | GCG AAC TGC GAT AAG TAA TGT | 57 | 111 | 57 |
Antisense | GCA ATG ACG CTC GGA CAG | 58 | |||
C. cladosporoides | Sense | GCG TCA TTT CAC CAC TCA AG | 58 | 128 | 57 |
Antisense | CTC CCG AAC ACC CTT TAG | 56 | |||
Penicillium chrysogenum | Sense | CTG TCC GAG CGT CAT TGC | 58 | 137 | 57 |
Antisense | CAG AGC GGG TGA CAA AGC | 58 | |||
S. chartarum | Sense | GAG TAG TTT GGG AAT GCT GC | 58 | 174 | 57 |
Antisense | GCC CAA GTC TGG TCA TAA AC | 58 | |||
β-Tubulin | |||||
A. alternata | Sense | GCG AAC AAC AGA GAA CAA CAG | 54 | 143 | 55 |
Antisense | AAA GGC AGC ACC GAT TTG G | 56 | |||
A. fumigatus | Sense | GCT GGA GCG TAT GAA CGT C | 56 | 165 | 55 |
Antisense | GAC AGC GTC CAT GGT ACC | 55 | |||
A. versicolor | Sense | GCG AAC TGC GAT AAG TAA TGT | 55 | 108 | 55 |
Antisense | GCA ATG ACG CTC GGA CAG | 56 | |||
C. cladosporoides | Sense | GCA TAC ACC GAT TGA CAA C GCC | 55 | 121 | 55 |
Antisense | TTG GGA GTT CAT ACC | 56 | |||
P. chrysogenum | Sense | CGA TGG CGA TGG ACA GTA AG | 55 | 131 | 55 |
Antisense | ACT CAC ATG GTT GAA GTA GAC G | 55 | |||
S. chartarum | Sense | ACC TCT CGG CTC ACA ATT TC | 55 | 165 | 55 |
Antisense | TTG TTG CCA GCA CCA GAC | 56 |
Tm, Temperature.