Table 1.
Primer sequences for the studied genes
| Biosyntetic pathway | Gene | Primers | Annealing temperature (°C) | GenBank accession no. |
|---|---|---|---|---|
| Flavonoids | Chalcone isomerase (CHI) | F: TTGTTCTGATGGCCTAATGG | 58 | DY263683 |
| R: AAAGGCTGTCACCGATGAAT | 58 | |||
| Chalcone synthase (CHS) | F: GATGTTGGCCGAGTAATGCT | 60 | CV885475 | |
| R: ATGCCAGGTCCAAAAGCTAA | 59 | |||
| Flavonol synthase (FLS) | F: GGAGGTGGAGAGGGTCCAAG | 59 | AB011796 | |
| R: GGGCCACCACTCCAAGAGC | 61 | |||
| Flavonoid 3'-hydroxylase (F3'H) | F: CTCGAGCCTTCCTCAAAACC | 60 | HQ634392 | |
| R: AACAAGCACAATCCCCATTC | 57 | |||
| Dihydroflavonol 4-reductase (DFR) | F: CTGGGTTTATCGGTTCATGG | 60 | DQ084722 | |
| R: TCCACAGCACCTGTGAACAT | 60 | |||
| Acids | Malic enzyme (EMA) | F: ACATGACGACATGCTTCTGG | 58 | CB417399 |
| R: CGTAGCCACGCCTAGTTCAT | 60 | |||
| Malate dehydrogenase (MDH) | F: ATGGCCGCTACATCAGCTAC | 60 | DQ901430 | |
| R: TGCAACCCCCTTTTCAATAC | 59 | |||
| Aconitase (ACO) | F: AAGCCATGGGTCAAAACAAG | 59 | AF073507 | |
| R: GATTTCCCAGTGTCGGTTGT | 59 | |||
| Vacuolar citrate/H+ symporter (TRPA) | F: GGCGCCACTCCTACCTTCCC | 62 | EF028327 | |
| R: CGGTCATTGAAGAGTGCTCCCC | 60 | |||
| Sugars | Acid invertase (INVA) | F: ATTGCGGATGTGAAGAAAGG | 56 | AB074885 |
| R: TTTGCCATGCTTTGAGTGAG | 56 | |||
| Phosphoenolpyruvate carboxylase (PEPC) | F: AGCCAATGGGATTTCTGACA | 60 | EF058158 | |
| R: GCCAAGCCACACAGGTAAAT | 60 | |||
| Phosphofructokinase (PKF) | F: CGCCGACCTCAGTCCCGTC | 63 | AF095520 | |
| R: GCTGCACGCCCCATAAGCCG | 64 | |||
| Carotenes | 1-Deoxyxylulose 5-phosphate synthase (DXS) | F: GGCGAGGAAGCGACGAAGATGG | 62 | DN959423 |
| R: GGATCAGAACTGGCCCTGGCG | 62 | |||
| Phytoene synthase (PSY) | F: GCTCGTTGATGGGCCTAATGC | 59 | AB037975 | |
| R: CGGGCGTAAGAGGGATTTTGC | 59 | |||
| β-Carotene hydroxylase (HYB) | F: AGCCCTTCTGTCTCCTCACA | 59 | AF315289 | |
| R: CCGTGGAATTTATCCGAGTG | 59 | |||
| Lycopene β-cyclase 2 (LCY2) | F: GCATGGCAACTCTTCTTAGCCCG | 60 | FJ516403 | |
| R: AGCTCGCAAGTAAGGCCATTCC | 61 | |||
| Lycopene β-cyclase (LCYB) | F: GAATTCTTGCCCCAAGTTCA | 60 | AY16696 | |
| R: TATGGGCCACAAATCTTTCC | 59 | |||
| 9-cis-Epoxy hydroxy carotenoid dyoxygenase 3 (NCED3) | F: GCAGTCAAATTCAACAAAGG | 55 | DQ309332 | |
| R: AATCCCAAATCTTGACACCT | 55 | |||
| Aldarate and ascorbate metabolism | Ascorbate oxydase (AOC) | F: TCAGTGAGAACCCTAAAGC | 58 | DY293375 |
| R: CAGTACAACCCCAGTAAGC | 60 | |||
| Ascorbate peroxidase (LAPX) | F: CAGCGGGGACTTATGACG | 58 | EU719653 | |
| R: GCCCTCCGGTAACTTCAAC | 59 | |||
| Cellular Detoxification | MRP-like ABC transporter (MRP4) | F: AGAAGCAGCATGGAAGATGG | 60 | CD574223 |
| R: CCGATCGGTTGGCATACTC | 62 | |||
| Cation chloride cotransporter (CCC1) | F: GCAGCTTGCTACCTACATTGAC | 63 | FN662480 | |
| R: ACTGAACTCCACATCCCAAAAG | 61 | |||
| High-affinity K+ transporter 1 (HKT1) | F: GTCCATGGAGAAAAAGAACC | 58 | DY297409 | |
| R: TGCTAGTGTCCGTGAAGAAG | 60 | |||
| NADH kinase (NADK2) | F: TGCAGAGACAAGATATTCCC | 58 | DN619491 | |
| R: ATGTGAGGTGAGAAATCCC | 58 | |||
| Salt tolerance | Aquaporin PIP1A (PIP1) | F: GACACTCGGCCTGTTCTTG | 62 | CK938271 |
| R: TCCGGTAATTGGGATGGTAG | 60 | |||
| Salt overly sensitive 1 (SOS1) | F: ACCAGTCAGACAACCATTTG | 55 | DN959478 | |
| R: CCAATTAGCACCTCATAGAGAC | 58 | |||
| Sucrose and starch metabolism | Trehalose-6-phosphate synthase (TSC) | F: TGCAGAACCTGTAATGAAGC | 58 | FC875388 |
| R:CTGGTAGGATGCCGACTTAG | 62 |
* AT, annealing temperature (°C).
† GBA, GenBank accession number.