Comparison of chiloglottone 1 levels after a 2-min flash of sunlight compared with continuous sunlight in previously depleted flowers of C. trapeziformis and C. seminuda. NA = not applicable because the depleted treatment was not formally included in the C. trapeziformis experiment. Instead the dashed line shows the average chiloglottone 1 level in depleted plants (4·4 ng per callus, n = 27 for C. trapeziformis). Error bars are standard error of the mean based on pooled estimate of error variance. Labelled columns not connected by the same letter are deemed significantly different at P < 0·05, based on a Tukey–Kramer HSD test. Outcomes of ANOVA: for C. trapeziformis F3,18 = 9·5, P < 0·0006; for C. seminuda callus F5,27 = 4·9, P < 0·002; for C. seminuda sepals F5,31 = 10·1, P < 0·0001.