Fig. 5.

Number and area of cells in the root tip during soybean seed germination. Seeds were treated with distilled water, 25 mm N-acetylcysteine (NAC), 25 mm NAC + 300 p.p.m. ethephon, or 25 mm NAC + 100 mm H₂O₂. (A) Staining of the embryonic axis by toluidine blue. (B) Trace of cells in the root tip. (C) Total root tip area, (D) number of cells and (E) cell area of the root tip, based on traces that were measured in ImageJ software. Bars with different letters differ significantly (P < 0·05, Tukey's test, n = 5).